Composite

Part:BBa_K5236022:Design

Designed by: Allen Yang   Group: iGEM24_BASIS-China   (2024-10-01)


pET28a-pelB-BhrPETase(M57L)-6xHis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 308
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 218
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 912


Design Notes

For expressing the sequence, signal peptides and lac operator are suggested to consider when designing a pathway.


Source

It came from AI

References